Zhang Lab Overview

RUNX1: Hematopoietic Transcription Factor

The hematopoietic master regulator RUNX1 in transcription, protein-protein interactions and RNA processing

RUNX1-ETO: Oncogenic Fusion Protein

RUNX1 fusion to ETO and its role in leukemogenesis

RNA Processing in Hematological Disease

Aberrant RNA splicing, polyadenylation and R-loops in disease

ISG15 and Related Modifications in Cancer Immunity

ISG15 protein modification (ISGylation) as an immune response and its role in cancer

USP18 in Protein Modification and Interferon Signaling

USP18's role in interferon-initiated cell-signaling and cancer.

Recent Publications

·         Chen L, Chen JY, Huang YJ, Gu Y, Qiu J, Qian H, Shao C, Zhang X, Hu J, Li H, He S, Zhou Y, Abdel-Wahab Omar, Zhang DE, Fu XD. Augmented R-loop is a Unifying Mechanism for Myelodysplastic Syndromes Induced by High Risk Splicing Factor Mutations (* co-corresponding). Mol Cell. 2018 69:421-425.

·         Arimoto KI*, Löchte S*, Stoner SA, Burkart C, Zhang Y, Miyauchi S, Wilmes S, Fan JB, Heinisch JJ, Li Z, Yan M, Pellegrini S, Colland F, Piehler J*, Zhang DE*. STAT2 is an essential adaptor in USP18-mediated suppression of type I interferon signaling (* co-first or co-corresponding authors). Nat Struct Mol Biol. 2017. 24:279-89.

·         Weng S, Matsuura S, Mowery C, Stoner SA, Lam K, Ran D, Davis AG, Lo MC, Zhang DE. Restoration of MYC-repressed targets mediates the negative effects of GM-CSF on RUNX1-ETO leukemogenicity. Leukemia 2017 31:159-169.

·         Qiu J, Zhou B, Thol F, Zhou Y, chen L, shao C, DeBoever C, Hou J, Li H, Chaturvedi A, Ganser A, Bejar R, Zhang DE*, Fu SD*, Heuser M*. Distinct splicing signatures affect converged pathways in myelodysplastic syndrome patients carrying mutations in different splicing regulators (co-corresponding). RNA 2016 22:1535-49.

·         Lam K, Muselman A, Du R, Yan M, Matsuura S, Zhang DE. Loss of RUNX1 function results in enhanced granulocyte-colony-stimulating factor-mediated mobilization. Blood Cancer J. 2016 6:e407.

·         Fan JB, Miyauchi-Ishida S, Arimoto KI, Yan M, Liu C, Gyorffy B, Zhang DE. Type I IFN induces protein ISGylation to enhance cytokine expression and augments colonic inflammation. PNAS, 2015, 112:14313-8.

·         Fan JB, Arimoto KI, Motamedchaboki K, Yan M Wolf DA, Zhang DE. Identification and characterization of a novel ISG15-ubiquitin mixed chain and its role in regulating protein homeostasis. Scientific Report 2015, 5:12704.

·        Komeno Y, Huang YJ, Qiu J, Lin L, Xu Y, Zhou Y, Chen L, Monterroza DD, Li H, DeKelver RC, Yan M, Fu XD, Zhang DE. SRSF2 Is Essential for Hematopoiesis, and Its Myelodysplastic Syndrome-Related Mutations Dysregulate Alternative Pre-mRNA Splicing. Mol Cell Biol. 2015. 35(17):3071-82

·        Komeno Y, Yan M, Matsuura S, Lam K, Lo MC, Huang YJ, Tenen DG, Downing JR, Zhang DE. Runx1 exon 6 related alternative splicing isoforms differentially regulate hematopoiesis in mice. Blood. 2014 123:3760-9.

·         Lam L, Muselman A, Du R, Harada Y, Scholl AG, Yan M, Matsuura S, Weng S, Harada H, Zhang DE. Hmga2 is a direct target gene of RUNX1 and regulates expansion of myeloid progenitors in mice. Blood. 2014. 124:2203-12.